A large-scale study on the effectiveness of a Bacillus subtilis Ch-13-based biofungicide against green mould disease and mushroom yield improvement

The aim of this study was to test a biofungicide based on Bacillus subtilis Ch-13 and its effectiveness in the control green mould disease cultivated mushroom comparison with fungicide prochloraz. Biofungicide impact yield were evaluated Agaricus bisporus after natural infection Trichoderma aggressivum commercial growing facility. An assay for testing microbial efficacy conducted two different procedures involving either three or split doses. highest statistically significant shown by prochloraz (71.43%), followed applied tree doses (53.57%), finally (45.46%). significantly improved an untreated Three applications B. strain enhanced larger extent than doses, although same final amount used both procedures. application increased total mass harvested mushrooms 8.41% compared control, 10.53% These results implied that should be applications: 30 ml (second day casing) + 15 (two weeks (after first flush, 20-25 days casing). further investigated regarding joint usage chemical fungicides procedures, as it showed remarkable characteristics terms promoting inhibiting spread mycopathogenic T. aggressivum.